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当前位置: 首页 > 产品中心 > Protein_purification_kit > NewEast/Rab5蛋白Q79L突变体[10117][25µg]/10117
商品详细NewEast/Rab5蛋白Q79L突变体[10117][25µg]/10117
NewEast/Rab5蛋白Q79L突变体[10117][25µg]/10117
NewEast/Rab5蛋白Q79L突变体[10117][25µg]/10117
商品编号: 10117
市场价: ¥4960.00
美元价: 2976.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白纯化试剂盒
公司分类: Protein_purification_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Catalog Number:  10117
Product Name:  Rab5 Protein Q79L mutant
Synonyms:  Member RAS oncogene family, RAB5A
Source:   Human, recombinant full length, His6-tag
Expression Host:   E. coli
Molecular Weight:   17 kDa
Purity:   >96% by SDS-PAGE
Introduction:   Rab5, consists of three isoforms, Rab5A, Rab5B, and Rab5C, is a small GTPase that is localized to early endosomes. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes.
Amino Acid Sequence    (16-184, Q79L)
NKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGLERY HSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQ SYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKN
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration: 1 mg/mL
Storage:   -80°C
Preparation Instructions:  Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab5 Q79L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
References:  1. Bucci, C. et al., Cell 70: 715-728, 1992. 2. Kinchen, J. M. et al., Nature 464: 778-782, 2010. 3. Kitano, M. et al., Nature 453: 241-245, 2008. 4. Lanzetti, L. et al., Nature 429: 309-314, 2004. 5. Lanzetti, L. et al., Nature 408: 374-377, 2000. 6. Miaczynska, M. et al., Cell 116: 445-456, 2004. 7. Ohya, T. et al., Nature 459: 1091-1097, 2009. 8. Otomo, A. et al., Hum. Molec. Genet. 12: 1671-1687, 2003. 9. Rousseau-Merck, M. F. et al., Hum. Genet. 86: 350-354, 1991. 10. Stenmark, H. et al., Cell 83: 423-432, 1995. 11. Xiao, G.-H. et al., J. Biol. Chem. 272: 6097-6100, 1997. 12. Zahraoui, A. et al., J. Biol. Chem. 264: 12394-12401, 1989.
Datasheet:  

品牌介绍
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