4000-520-616
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4000-520-616
当前位置: 首页 > 产品中心 > Other_kits > NewEast/Arl1激活分析试剂盒[82901][20次分析]/82901
商品详细NewEast/Arl1激活分析试剂盒[82901][20次分析]/82901
NewEast/Arl1激活分析试剂盒[82901][20次分析]/82901
NewEast/Arl1激活分析试剂盒[82901][20次分析]/82901
商品编号: 82901
市场价: ¥14740.00
美元价: 8844.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductHighlight

  • Configuration-specificmonoclonalantibodythatrecognizesArl1-GTP,butnotArl1-GDP
  • TheonlyavailableArl1activationassaykitdirectlymeasuresthelevelofArl1-GTP
  • Providingshorterexperimentaltime
  • CouldbeusedtomonitortheactivationofArl1incellsandintissuesbyimmunohistochemistry

ProductDescription

    Arf-likeprotein1(Arl1)isamemberoftheArffamilyofregulatoryGTPases,withintheRassuperfamilyofGTPases,andwithhighlyconservedorthologsthroughouteukaryotes.Arl1isessentialforearlyembryonicdevelopmentinDrosophilaandinCaenorhaBDitiselegans.Arl1ismostsimilarinprimarysequence,cellularlocation,andfunction(regulationofmembranetraffic)toArf1-6andevensharesseveralcommonbindingpartners.InadditiontoitsfunctioninmembranetrafficattheGolgi/trans-Golginetwork,therearereportsindicatingapossIBLeroleforArl1inionhomeostasisinyeast.

    CurrentlythereisnodirectassaytomeasuretheactivationofArl1GTPases.

    NewEastBiosciencesArl1ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesArl1-GTP,butnotArl1-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

    Theseanti-Arl1-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofArl1incellsandintissuesbyimmunohistochemistry.

    NewEastBiosciencesArl1ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofArl1.Eachkitprovidessufficientquantitiestoperform20assays.

    AssayPrinciple

      NewEastBiosciencesArl1ActivationAssayKitbasesontheconfiguration-specificanti-Arl1-GTPmonoclonalantibodytomeasuretheactiveArl1-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngArl1activationassays.Briefly,anti-activeArl1mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingArl1-GTP.TheboundactiveArl1willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveArl1willbedetectedbyimmunoblotanalysisusinganti-Arl1rabbitpolyclonalantibodyoranti-Arl1mousemonoclonalantibody.