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ProductDescriptionArf-likeprotein1(Arl1)isamemberoftheArffamilyofregulatoryGTPases,withintheRassuperfamilyofGTPases,andwithhighlyconservedorthologsthroughouteukaryotes.Arl1isessentialforearlyembryonicdevelopmentinDrosophilaandinCaenorhaBDitiselegans.Arl1ismostsimilarinprimarysequence,cellularlocation,andfunction(regulationofmembranetraffic)toArf1-6andevensharesseveralcommonbindingpartners.InadditiontoitsfunctioninmembranetrafficattheGolgi/trans-Golginetwork,therearereportsindicatingapossIBLeroleforArl1inionhomeostasisinyeast. CurrentlythereisnodirectassaytomeasuretheactivationofArl1GTPases. NewEastBiosciencesArl1ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesArl1-GTP,butnotArl1-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Theseanti-Arl1-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofArl1incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesArl1ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofArl1.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesArl1ActivationAssayKitbasesontheconfiguration-specificanti-Arl1-GTPmonoclonalantibodytomeasuretheactiveArl1-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngArl1activationassays.Briefly,anti-activeArl1mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingArl1-GTP.TheboundactiveArl1willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveArl1willbedetectedbyimmunoblotanalysisusinganti-Arl1rabbitpolyclonalantibodyoranti-Arl1mousemonoclonalantibody.
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品牌分类
10μg纯化g蛋白
1x96井生物标志物
100微升单克隆抗体
100ul纯化G蛋白
突变癌基因100微升抗体
96井营地和cGMP环境影响评价工具包
30微升活性单克隆抗体
100微升生物标志物
30微升生物标志物
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