ProductHighlight- BasedontheuniquemousemonoclonalAnti-cAMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cAMPantibody(allotherkitsarebasedonpolyclonalanti-cAMPantibodies).
- Nomorehazardouschemical(aceticanhydride)foryourcAMPassayThemonoclonalAnti-cAMPantibodyhassimilaraffinitiesfornon-acetylatedcAMPandacetylatedcAMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay.
- SupersensitivityandselectivityThemonoclonalAnti-cAMPantibodydisplays>108foldofselectivityovercGMP,ATP,andothernucleosideanalogues.TheAnti-cAMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cAMPantibodiesonthemarket.
- Shortestassaytimeandhigh-throughputformatTheAnti-cAMPEIAKitisthebestchoicefordrugscreenings.
ProductDescriptionAdenosine3",5"-cyclicmonophosphate(cyclicAMP;cAMP)modulatesvariousphysiologicalfunctionssuchascardiovascularBIOLOGy,learningandmemory,olfaction,immuneresponse,asthmaandkidneyfunction(1,2).cAMPisproducedfromATPbyadenylylcyclasesandisdegradedbyphosphodiesterases.StimulationofadenylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcAMPconcentrations.Blockersofadenylylcyclase-activatingreceptorsandinhibitorsofthecAMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,blockingagentsforcAMP-increasingbeta-adrenergicreceptors(beta-blockers)areusedfortreatingabnormalheartrhythms,highbloodpressure(hypertension),myocardialinfarctionandheartfailure.InhibitorsofcAMPspecificphosphodiesterasetypes2and4arebeingtestedforcognitionenhancement. ToscreenforinhibitorsorstimulatorsofcellularcAMPlevels,itisessentialtohaveasensitive,selectiveandreproducIBLemethodtomeasurethecAMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds. CurrentlyavailableotherELISAkitsmeasuringcAMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cAMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cAMPantibodiesdisplaycertaincross-reactivitywithATP.GiventhatATPisthesubstrateforthecAMPproduction,itisverydesirabletohaveanantibodywithhighspecificitytowardscAMPoverATP. NewEastBiosciencescAMPELISAkitisbasedontheuniquemousemonoclonalanti-cAMPantibody.Thismonoclonalanti-cAMPantibodydisplays>108foldofselectivityoverATP,cGMP,andothernucleosideanalogues.NewEastBiosciencescAMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cAMPantibodies.Ourmonoclonalanti-cAMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun. FurThermore,whilepolyclonalanti-cAMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcAMPthannon-acetylatedcAMP,NewEastBiosciencesmonoclonalanti-cAMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcAMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescAMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment. PrincipleOutlineNewEastBiosciencescAMPELISAKitisacompetitiveimmunoassaytomeasurethecAMPlevels,eitherfromcellextractsorfrominvitroadenylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cAMPincellextractsorininvitroadenylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cAMPantibodyinthepresenceoffixedamountsofcAMP-conjugatedhorse-rADIshperoxidaseoralkalinephosphatase.KnownamountsofcAMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nmor405nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcAMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcAMPinsamplesbasedonthecurvefromthecAMPstandards. FAQs ProductManual AssayLayoutSheet Publications:1. Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1 PflugersArch.2012Feb;463(2):257-68 | 2. Antiarrhythmiceffectofprolongedmorphineexposureisaccompaniedbyalteredmyocardialadenylylcyclasesignalinginrats PharmacolRep.2012;64(2):351-9 | 3. UncouplingofM1muscarinicreceptor/G-proteininteractionbyamyloidbeta(1-42) Neuropharmacology.2013Apr;67:272-83 | 4. SubendocardialIncreaseinReactiveOxygenSpeciesProductionAffectsRegionalContractileFunctioninIschemicHeartFailure AntioxidRedoxSignal.2013Mar20;18(9):1009-20 | 5. Heterogeneityinrelaxationofdifferentsizedporcinecoronaryarteriestonitrovasodilators:roleofPKGandMYPT1 PflugersArch.2012Feb;463(2):257-68 | 6. Structuralbasisofanthraxedemafactorneutralizationbyaneutralizingantibody BiochemicalandBiophysicalResearchCommunicationsVolume417,Issue1,6January2012,Pages324–329 | 7. TransgenicrescueofdefectiveCd36enhancesmyocardialadenylylcyclasesignalinginspontaneouslyhypertensiverats PflügersArchiv-EuropeanJournalofPhysiologyOctober2013,Volume465,Issue10,pp1477-1486 | 8. OpposingHDAC4nuclearfluxesduetophosphorylationbyβadrenergicactivatedPKAorbyactivityorEpacactivatedCaMKIIinskeletalmusclefibres TheJournalofPhysiologyVolume591,Issue14,pages3605–3623,July2013 | 9. SexisamajordeterminantofneuronaldysfunctioninNeurofibromatosisType1 AnnalsofNeurologyVolume75,Issue2,pages309–316,February2014 | 10. cAMP–PKAinhibitionofSK3channelreducedbothCa2+entryandcancercellmigrationbyregulationofSK3–Orai1complex PflügersArchiv-EuropeanJournalofPhysiologyOctober2014,Volume466,Issue10,pp1921-1932 | 11. Immunomodulatingeffectsofcasein-derivedpeptideQEPVLandQEPVonlymphocytesinvitroandinvivo FoodFunct.,2014,5,2061-2069 | 12. AgoNIST-DependentAnd-IndependentDopamine-1-LikeReceptorSignallingDifferentlyRegulatesDownstreamEffectors FEBSJournalVolume281,Issue21,pages4792–4804,November2014 | 13. Alterationofvascularreactivityinheartfailure:Roleofphosphodiesterasestype3and4 BritishJournalofPharmacologyVolume171,Issue23,pages5361–5375,December2014 | 14. Ischemia/Reperfusion-InducedCHOPExpressionPromotesApoptosisandImpairsRenalFunctionRecovery:TheRoleofAcidosisandGPR4 PLoSOne.2014Oct24;9(10):e110944 | 15. Comparativestudyofsomatostatin-humanserumalbuminfusionproteinsandnaturalsomatostatinonreceptorbinding,internalizationandactivation PLoSOne.2014Feb27;9(2):e89932 |
品牌介绍
美国NewEastBio专注于生命科学和生物技术领域,主要产品有抗体、小分子、ELISA试剂盒、蛋白等,与世界著名高校和研究所、世界大制药公司、生物科技公司和医院等客户建立了长期稳定的合作关系,产品销往全球。 美国NewEastBio,负责美国品牌NewEastBio(www.neweastbio.com)产品在中国市场的推广、销售及售后服务。致力于为客户提供一系列高质量和有绝对价格竞争力的美国NewEastBio原装进口产品。并提供专业的技术服务:抗体定制,动物实验等
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