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BRaf (V600E)

来自 : 发布时间：2024-05-04

货号：	26039
数量：	大量
抗原来源：	鼠
宿主：	mouse
抗体英文名：	Anti-B-Raf(V600E) Mouse Monoclonal Antibody
是否单克隆：	否
抗体名：	BRaf (V600E)
靶点：	BRaf (V600E)
浓度：	0.5 mg/ml
应用范围：	ELISA、WB、IHC、IP
适应物种：	脊椎动物
保存条件：	-20℃冻存
形态：	液体
亚型：	IgG
规格：	100μl

B-Raf (V600E) [26039][100 µL]

Catalog Number: 26039

Gene Symbol: B-Raf

Description: Anti-B-Raf(V600E) Mouse Monoclonal Antibody

Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:2000 IHC: 1:10-1:100

Concentration: 0.5 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 59% glycerol

Species Reactivity: B-Raf(V600E) antibody recognizes mutated, but not wild-type, B-Raf(V600E) proteins.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf(V600E) (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat # 26039).

Immunofluorescence:

Immunofluorescence of cells expressing B-Raf (V600E) protein with anti-B-Raf (V600E) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf WT plasmid (left column) or pCDNA3-GFP-B-Raf V600E plasmid (right column), then fixed and stained with anti- B-Raf (V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti-B-Raf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

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Publications:

1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma Mod Pathol. 2013 Mar;26(3):414-20.

2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733

3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma human pathology. February 2015Volume 46, Issue 2, Pages 169–175

4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570

5. BRAF V600E Mutation-Specific Antibody: A Review Seminars in Diagnostic Pathology

温馨提示：不可用于临床治疗。

本文链接： http://neweastbio.immuno-online.com/view-1562382010.html

发布于： 2024-05-04 阅读（）

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